Diagnosis
Morphological – adult
A medium sized species. Features include:
- face fulvous with a pair of large oval black spots
- postpronotal lobes and notopleura yellow
- scutum dull black; lateral postsutural vittae present
- medial postsutural vitta absent
- mesopleural stripe extending to anterior npl. seta dorsally
- scutellum yellow
- wing with a narrow fuscous costal band overlapping R2+3 and expanding into a small spot around apex of R4+5, a medium width fuscous anal streak
- cells bc and c colourless; microtrichia in outer corner of cell c only
- all abdominal terga entirely dark orange-brown, posterior lobe of male surstylus short
- female with apex of aculeus trilobed (Drew and Romig 2013).
Morphological – larvae
See White and Elson-Harris 1992 p. 210.
Molecular
DNA barcoding
Diagnostic BOLD reference data available.
DDOSTs2 data
B. latifrons LTF003 Classic specimen DDOSTs2
B. latifrons LTF004 Classic specimen DDOSTs2
EIF3L data
B. latifrons LTF003 Classic specimen EIF3L
B. latifrons LTF004 Classic specimen EIF3L
FCOI data
B. latifrons LTF003 Classic specimen FCOI
B. latifrons LTF004 Classic specimen FCOI
POP4 data
B. latifrons LTF003 Classic specimen POP4
RPA2 data
B. latifrons LTF003 Classic specimen RPA2
B. latifrons LTF004 Classic specimen RPA2
PCR-RFLP Test 1
BsrI: 600, 200
HinfI: Does not cut
HhaI: 600, 190
Sau3AI: Does not cut
SnaBI: Does not cut
SspI: Does not cut
Vspl: Does not cut
Approximate ITS1 fragment length – gel: 770 bp.
PCR-RFLP Test 2
Sau3A produces a diagnostic pattern. Other useful enzymes DraI, NcoI and RsaI
Sau3A: 760, 730, 110